Measurement of cell volume using in-line digital holography | OUP Journals & Magazine | IEEE Xplore

Measurement of cell volume using in-line digital holography

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Abstract:

The measurement of the volume of blood cells is important for clinical diagnosis and patient management. While digital holography microscopy has been used to obtain such ...Show More

Abstract:

The measurement of the volume of blood cells is important for clinical diagnosis and patient management. While digital holography microscopy has been used to obtain such information, previous off-axis setups usually involve a separated reference beam and are thus not very easy to implement. Here, we use the simple in-line Gabor setup without separation of a reference beam to measure the shape and volume of cells mounted on glass slides. Inherent to the in-line holograms, the reconstructed phase of the object is affected by the virtual image noise, producing errors in the cell volume measurement. We optimized our approach to use a single hologram without phase retrieval, increasing distance between cell and hologram plane to reduce the measurement error of cell volume to less than 6% in some instances. Therefore, the in-line Gabor setup can be a useful and simple tool to obtain volumetric and morphologic cellular information.
Published in: Microscopy ( Volume: 70, Issue: 4, August 2021)
Page(s): 333 - 339
Date of Publication: 18 October 2021

ISSN Information: