Abstract:
Multiparameter spectrophotometer instruments based on the radiative transfer (RT) theory differ fundamentally from conventional ones in their ability to determine absorpt...Show MoreMetadata
Abstract:
Multiparameter spectrophotometer instruments based on the radiative transfer (RT) theory differ fundamentally from conventional ones in their ability to determine absorption and scattering coefficients and anisotropy factor. Ease of use and robustness of inverse solutions are of necessity for general-purpose applications. We have developed a new instrument platform design incorporating a compact three-photodiode detection configuration without integrating spheres, four-channel signal-processing unit, and Monte Carlo (MC) simulation-based software for accurate and rapid inverse solutions. To verify the platform design, two sphere suspension samples of different size distributions were measured and RT parameters were determined by a sample model with the Henyey and Greenstein (HG) phase function between 460 and 1000 nm. System validation is achieved by comparison of the RT parameters and spectral dependence against the results by Mie theory for single-sized spheres of nominal diameter values. Five wavelengths from 560 to 960 nm were selected for quantitative evaluation of the robustness of the HG sample model and two Mie theory-based sample models assuming light scattering by independent and single spheres. We found that only the HG-based sample model yields high accuracy and strong robustness for inverse determination of RT parameters even for the cases of sphere suspensions in which the assumptions of sample uniformity and scattering by independent and single spheres are not satisfied. These results demonstrate that the new platform with MC simulation-based inverse algorithm combined with the HG sample model is capable of robust and rapid measurement of RT parameters for turbid samples with optical thickness up to 30.
Published in: IEEE Transactions on Instrumentation and Measurement ( Volume: 70)
Funding Agency:
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
Department of Physics, East Carolina University, Greenville, NC, USA
Key Laboratory of Hunan Province on Information Photonics and Optical Communication, Hunan Institute of Science and Technology, Yueyang, China
School of Physics and Electronic Science, Hunan Institute of Science and Technology, Yueyang, China
Peng Tian received the B.S. degree from Hunan Normal University, Changsha, China, in 2000 and the M.S. and Ph.D. degrees in electronic science and technology from the Huazhong University of Science and Technology of China, Wuhan, China, in 2006 and 2011, respectively.
He is currently an Associate Professor with the Hunan Institute of Science and Technology, Yueyang, China, and has visited the Biomedical Laser Laboratory, D...Show More
Peng Tian received the B.S. degree from Hunan Normal University, Changsha, China, in 2000 and the M.S. and Ph.D. degrees in electronic science and technology from the Huazhong University of Science and Technology of China, Wuhan, China, in 2006 and 2011, respectively.
He is currently an Associate Professor with the Hunan Institute of Science and Technology, Yueyang, China, and has visited the Biomedical Laser Laboratory, D...View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
School of Physics and Electronic Science, Hunan Institute of Science and Technology, Yueyang, China
Yonghong Qin received the B.S. degree in electronic and information engineering from the Hunan Institute of Science and Technology, Yueyang, China, in 2018, where she is currently pursuing the M.S. degree in information and communication engineering.
Her current research interests are in the optical design and light scattering signal measurement with multiparameter spectrophotometry and parallel Monte Carlo simulations by ...Show More
Yonghong Qin received the B.S. degree in electronic and information engineering from the Hunan Institute of Science and Technology, Yueyang, China, in 2018, where she is currently pursuing the M.S. degree in information and communication engineering.
Her current research interests are in the optical design and light scattering signal measurement with multiparameter spectrophotometry and parallel Monte Carlo simulations by ...View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
School of Physics and Electronic Science, Hunan Institute of Science and Technology, Yueyang, China
Lin Zhao received the M.S. degree in computer application technology from the Huazhong University of Science and Technology, Wuhan, China, in 2007, and the Ph.D. degree in control science and engineering from Central South University, Changsha, China, in 2017.
He is currently an Associate Professor with the Hunan Institute of Science and Technology, Yueyang, China, and conducts research in optical imaging, artificial intel...Show More
Lin Zhao received the M.S. degree in computer application technology from the Huazhong University of Science and Technology, Wuhan, China, in 2007, and the Ph.D. degree in control science and engineering from Central South University, Changsha, China, in 2017.
He is currently an Associate Professor with the Hunan Institute of Science and Technology, Yueyang, China, and conducts research in optical imaging, artificial intel...View more
Department of Physics, East Carolina University, Greenville, NC, USA
Stephen M. Mutisya received the B.S. and M.S. degrees in physics from Virginia Commonwealth University, Richmond, VA, USA, in 2009 and 2011, respectively. He is currently a doctoral graduate student in the Department of Physics, East Carolina University, Greenville, NC, USA.
He also works as a Metrology Engineer with Intel Corporation, Albuquerque, NM, USA. His research specializes in the development of a new spectrophotom...Show More
Stephen M. Mutisya received the B.S. and M.S. degrees in physics from Virginia Commonwealth University, Richmond, VA, USA, in 2009 and 2011, respectively. He is currently a doctoral graduate student in the Department of Physics, East Carolina University, Greenville, NC, USA.
He also works as a Metrology Engineer with Intel Corporation, Albuquerque, NM, USA. His research specializes in the development of a new spectrophotom...View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
Department of Physics, East Carolina University, Greenville, NC, USA
Key Laboratory of Hunan Province on Information Photonics and Optical Communication, Hunan Institute of Science and Technology, Yueyang, China
School of Physics and Electronic Science, Hunan Institute of Science and Technology, Yueyang, China
Jiahong Jin received the B.S. degree in applied mathematics and the M.S. degree in optics from Sun Yat-sen University, Guangzhou, China, in 2007 and 2014, respectively. He is currently pursuing the Ph.D. degree in biomedical physics with the Department of Physics, East Carolina University, Greenville, NC, USA.
He joined the Physics Faculty, Hunan Institute of Science and Technology, Yueyang, China, in 2014, and the Institu...Show More
Jiahong Jin received the B.S. degree in applied mathematics and the M.S. degree in optics from Sun Yat-sen University, Guangzhou, China, in 2007 and 2014, respectively. He is currently pursuing the Ph.D. degree in biomedical physics with the Department of Physics, East Carolina University, Greenville, NC, USA.
He joined the Physics Faculty, Hunan Institute of Science and Technology, Yueyang, China, in 2014, and the Institu...View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
Department of Physics, East Carolina University, Greenville, NC, USA
Jun Q. Lu received the B.S. and M.S. degrees from Nankai University, Tianjin, China, in 1983 and 1986, respectively, and the Ph.D. degree from the University of California at Irvine, Irvine, CA, USA, in 1991, all in physics.
She is currently with the Faculty of Physics, East Carolina University, Greenville, NC, USA. Her research areas include theoretical modeling, numerical simulations, and machine learning-based analysis ...Show More
Jun Q. Lu received the B.S. and M.S. degrees from Nankai University, Tianjin, China, in 1983 and 1986, respectively, and the Ph.D. degree from the University of California at Irvine, Irvine, CA, USA, in 1991, all in physics.
She is currently with the Faculty of Physics, East Carolina University, Greenville, NC, USA. Her research areas include theoretical modeling, numerical simulations, and machine learning-based analysis ...View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
Department of Physics, East Carolina University, Greenville, NC, USA
Xin-Hua Hu received the B.S. and M.S. degrees in physics from Nankai University, Tianjin, China, in 1982 and 1985, respectively, the M.S. degree in physics from Indiana University at Bloomington, Bloomington, IN, USA, and the Ph.D. degree in physics from the University of California at Irvine, Irvine, CA, USA, in 1991.
He joined the Physics Faculty, East Carolina University, Greenville, NC, USA, in 1995, and established th...Show More
Xin-Hua Hu received the B.S. and M.S. degrees in physics from Nankai University, Tianjin, China, in 1982 and 1985, respectively, the M.S. degree in physics from Indiana University at Bloomington, Bloomington, IN, USA, and the Ph.D. degree in physics from the University of California at Irvine, Irvine, CA, USA, in 1991.
He joined the Physics Faculty, East Carolina University, Greenville, NC, USA, in 1995, and established th...View more
Contents Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
Department of Physics, East Carolina University, Greenville, NC, USA
Key Laboratory of Hunan Province on Information Photonics and Optical Communication, Hunan Institute of Science and Technology, Yueyang, China
School of Physics and Electronic Science, Hunan Institute of Science and Technology, Yueyang, China
Peng Tian received the B.S. degree from Hunan Normal University, Changsha, China, in 2000 and the M.S. and Ph.D. degrees in electronic science and technology from the Huazhong University of Science and Technology of China, Wuhan, China, in 2006 and 2011, respectively.
He is currently an Associate Professor with the Hunan Institute of Science and Technology, Yueyang, China, and has visited the Biomedical Laser Laboratory, Department of Physics, East Carolina University, Greenville, NC, USA, from 2016 to 2017, where he is currently a Visiting Faculty. His research interests are in the spectroscopic measurement and imaging of scattered light signals and efficient algorithm on solving inversion turbidity problems based on Monte Carlo simulations.
Peng Tian received the B.S. degree from Hunan Normal University, Changsha, China, in 2000 and the M.S. and Ph.D. degrees in electronic science and technology from the Huazhong University of Science and Technology of China, Wuhan, China, in 2006 and 2011, respectively.
He is currently an Associate Professor with the Hunan Institute of Science and Technology, Yueyang, China, and has visited the Biomedical Laser Laboratory, Department of Physics, East Carolina University, Greenville, NC, USA, from 2016 to 2017, where he is currently a Visiting Faculty. His research interests are in the spectroscopic measurement and imaging of scattered light signals and efficient algorithm on solving inversion turbidity problems based on Monte Carlo simulations.View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
School of Physics and Electronic Science, Hunan Institute of Science and Technology, Yueyang, China
Yonghong Qin received the B.S. degree in electronic and information engineering from the Hunan Institute of Science and Technology, Yueyang, China, in 2018, where she is currently pursuing the M.S. degree in information and communication engineering.
Her current research interests are in the optical design and light scattering signal measurement with multiparameter spectrophotometry and parallel Monte Carlo simulations by GPU.
Yonghong Qin received the B.S. degree in electronic and information engineering from the Hunan Institute of Science and Technology, Yueyang, China, in 2018, where she is currently pursuing the M.S. degree in information and communication engineering.
Her current research interests are in the optical design and light scattering signal measurement with multiparameter spectrophotometry and parallel Monte Carlo simulations by GPU.View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
School of Physics and Electronic Science, Hunan Institute of Science and Technology, Yueyang, China
Lin Zhao received the M.S. degree in computer application technology from the Huazhong University of Science and Technology, Wuhan, China, in 2007, and the Ph.D. degree in control science and engineering from Central South University, Changsha, China, in 2017.
He is currently an Associate Professor with the Hunan Institute of Science and Technology, Yueyang, China, and conducts research in optical imaging, artificial intelligence, and computational modeling for optimal control of complex industrial processes.
Lin Zhao received the M.S. degree in computer application technology from the Huazhong University of Science and Technology, Wuhan, China, in 2007, and the Ph.D. degree in control science and engineering from Central South University, Changsha, China, in 2017.
He is currently an Associate Professor with the Hunan Institute of Science and Technology, Yueyang, China, and conducts research in optical imaging, artificial intelligence, and computational modeling for optimal control of complex industrial processes.View more
Department of Physics, East Carolina University, Greenville, NC, USA
Stephen M. Mutisya received the B.S. and M.S. degrees in physics from Virginia Commonwealth University, Richmond, VA, USA, in 2009 and 2011, respectively. He is currently a doctoral graduate student in the Department of Physics, East Carolina University, Greenville, NC, USA.
He also works as a Metrology Engineer with Intel Corporation, Albuquerque, NM, USA. His research specializes in the development of a new spectrophotometric method for turbidity characterization.
Stephen M. Mutisya received the B.S. and M.S. degrees in physics from Virginia Commonwealth University, Richmond, VA, USA, in 2009 and 2011, respectively. He is currently a doctoral graduate student in the Department of Physics, East Carolina University, Greenville, NC, USA.
He also works as a Metrology Engineer with Intel Corporation, Albuquerque, NM, USA. His research specializes in the development of a new spectrophotometric method for turbidity characterization.View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
Department of Physics, East Carolina University, Greenville, NC, USA
Key Laboratory of Hunan Province on Information Photonics and Optical Communication, Hunan Institute of Science and Technology, Yueyang, China
School of Physics and Electronic Science, Hunan Institute of Science and Technology, Yueyang, China
Jiahong Jin received the B.S. degree in applied mathematics and the M.S. degree in optics from Sun Yat-sen University, Guangzhou, China, in 2007 and 2014, respectively. He is currently pursuing the Ph.D. degree in biomedical physics with the Department of Physics, East Carolina University, Greenville, NC, USA.
He joined the Physics Faculty, Hunan Institute of Science and Technology, Yueyang, China, in 2014, and the Institute for Advanced Optics in 2016. He is conducting research in optical imaging of turbid materials and biological tissues and machine learning study on diffraction image data of cells.
Jiahong Jin received the B.S. degree in applied mathematics and the M.S. degree in optics from Sun Yat-sen University, Guangzhou, China, in 2007 and 2014, respectively. He is currently pursuing the Ph.D. degree in biomedical physics with the Department of Physics, East Carolina University, Greenville, NC, USA.
He joined the Physics Faculty, Hunan Institute of Science and Technology, Yueyang, China, in 2014, and the Institute for Advanced Optics in 2016. He is conducting research in optical imaging of turbid materials and biological tissues and machine learning study on diffraction image data of cells.View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
Department of Physics, East Carolina University, Greenville, NC, USA
Jun Q. Lu received the B.S. and M.S. degrees from Nankai University, Tianjin, China, in 1983 and 1986, respectively, and the Ph.D. degree from the University of California at Irvine, Irvine, CA, USA, in 1991, all in physics.
She is currently with the Faculty of Physics, East Carolina University, Greenville, NC, USA. Her research areas include theoretical modeling, numerical simulations, and machine learning-based analysis of light scattering data acquired from biological cells and turbid samples.
Jun Q. Lu received the B.S. and M.S. degrees from Nankai University, Tianjin, China, in 1983 and 1986, respectively, and the Ph.D. degree from the University of California at Irvine, Irvine, CA, USA, in 1991, all in physics.
She is currently with the Faculty of Physics, East Carolina University, Greenville, NC, USA. Her research areas include theoretical modeling, numerical simulations, and machine learning-based analysis of light scattering data acquired from biological cells and turbid samples.View more
Institute for Advanced Optics, Hunan Institute of Science and Technology, Yueyang, China
Department of Physics, East Carolina University, Greenville, NC, USA
Xin-Hua Hu received the B.S. and M.S. degrees in physics from Nankai University, Tianjin, China, in 1982 and 1985, respectively, the M.S. degree in physics from Indiana University at Bloomington, Bloomington, IN, USA, and the Ph.D. degree in physics from the University of California at Irvine, Irvine, CA, USA, in 1991.
He joined the Physics Faculty, East Carolina University, Greenville, NC, USA, in 1995, and established the Biomedical Laser Laboratory. As a Professor, he conducts and directs research programs in optical imaging, theoretical modeling, and machine learning study of light scattering by human cells, in turbid materials and biological tissues. He is also interested in the development of novel instrumentation of spectrophotometry and flow cytometry based on spectral measurement and imaging of light scattering signals.
Xin-Hua Hu received the B.S. and M.S. degrees in physics from Nankai University, Tianjin, China, in 1982 and 1985, respectively, the M.S. degree in physics from Indiana University at Bloomington, Bloomington, IN, USA, and the Ph.D. degree in physics from the University of California at Irvine, Irvine, CA, USA, in 1991.
He joined the Physics Faculty, East Carolina University, Greenville, NC, USA, in 1995, and established the Biomedical Laser Laboratory. As a Professor, he conducts and directs research programs in optical imaging, theoretical modeling, and machine learning study of light scattering by human cells, in turbid materials and biological tissues. He is also interested in the development of novel instrumentation of spectrophotometry and flow cytometry based on spectral measurement and imaging of light scattering signals.View more
