Effects of Steep Pulsed Electric Fields (SPEF) on Mitochondrial Transmembrane Potential of Human Liver Cancer Cell | IEEE Conference Publication | IEEE Xplore
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Effects of Steep Pulsed Electric Fields (SPEF) on Mitochondrial Transmembrane Potential of Human Liver Cancer Cell

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Yan Mi; Caixin Sun; Chenguo Yao; Chengxiang Li; Dengbin Mo; Liling Tang
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Abstract:

Mitochondrial plays an important role in apoptosis, and measuring the change of mitochondrial transmembrane potential (DeltaPsim) is a useful method for apoptosis. Exposi...Show More

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Abstract:

Mitochondrial plays an important role in apoptosis, and measuring the change of mitochondrial transmembrane potential (DeltaPsim) is a useful method for apoptosis. Exposing human liver cancer cell SMMC-7721 dyed with Rhodamine 123 to steep pulsed electric fields (SPEF), this paper observed the real-time change of DeltaPsim using laser scanning confocal microscope (LSCM) to study the apoptosis effect of SPEF. The experiment results showed that DeltaPsim decreased gradually in process of exposing to SPEF, SPEF with higher voltage (600 V) and shorter width (100 ns) could cause a quicker decrease than SPEF with lower voltage (200 V) and longer width (1.3 mus). Such phenomenon kept up even after canceling SPEF, this may lead to collapse of DeltaPsim and induce apoptosis with tremendous possibilities. The experiment results of flow cytometry (SMMC-7721 dyed with Annexin V-FITC) approved that SPEF could induce apoptosis markedly (P<0.01); SPEF with lower voltage (200 V) and longer width (1.3 mus) could induce apoptosis more effectively (P<0.01) than SPEF with higher voltage (600 V) and shorter width (100 ns). These experiment results supply possible mechanism and parameter selection basis for tumor treatment using SPEF.
Published in: 2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Date of Conference: 22-26 August 2007
Date Added to IEEE Xplore: 22 October 2007
ISBN Information:

ISSN Information:

PubMed ID: 18003335
DOI: 10.1109/IEMBS.2007.4353669
Conference Location: Lyon, France
References is not available for this document.
Contents

I. Introduction

Pulsed electric fields (PEF) is the focus of more and more biological or medical researchers for its outstanding nonthermal effects on cells and tissues in recent years. J. C. Weaver [1]–[3] found that cell membrane temporarily rearranged and pores in it came into being while exposing cell to PEF with electric field intensity of several kV/cm and duration of . Such alterations will make cell membrane more permeable to a large variety of hydrophilic molecules which can't enter into the cell in normal conditions. This transient reversible membrane alteration is termed as electroporation, i.e. reversible electrical breakdown (REB). Based on electroporation, G. A. Hofmann [4]–[6] brought forward electroporation therapy (EPT) combined with bleomycin for clinical tumor treatment. With increasing dose of PEF, irreversible electroporation happens to membrane and directly kills cancer cells. This phenomenon is termed as irreversible electrical breakdown (IREB). Now IREB is widely used to kill bacteria in liquid food and decontaminate liquids etc. Whereas there are few reports utilizing PEF to treat tumor without anticancer drug.

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