Abstract:
Mitochondrial plays an important role in apoptosis, and measuring the change of mitochondrial transmembrane potential (DeltaPsim) is a useful method for apoptosis. Exposi...Show MoreMetadata
Abstract:
Mitochondrial plays an important role in apoptosis, and measuring the change of mitochondrial transmembrane potential (DeltaPsim) is a useful method for apoptosis. Exposing human liver cancer cell SMMC-7721 dyed with Rhodamine 123 to steep pulsed electric fields (SPEF), this paper observed the real-time change of DeltaPsim using laser scanning confocal microscope (LSCM) to study the apoptosis effect of SPEF. The experiment results showed that DeltaPsim decreased gradually in process of exposing to SPEF, SPEF with higher voltage (600 V) and shorter width (100 ns) could cause a quicker decrease than SPEF with lower voltage (200 V) and longer width (1.3 mus). Such phenomenon kept up even after canceling SPEF, this may lead to collapse of DeltaPsim and induce apoptosis with tremendous possibilities. The experiment results of flow cytometry (SMMC-7721 dyed with Annexin V-FITC) approved that SPEF could induce apoptosis markedly (P<0.01); SPEF with lower voltage (200 V) and longer width (1.3 mus) could induce apoptosis more effectively (P<0.01) than SPEF with higher voltage (600 V) and shorter width (100 ns). These experiment results supply possible mechanism and parameter selection basis for tumor treatment using SPEF.
Published in: 2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Date of Conference: 22-26 August 2007
Date Added to IEEE Xplore: 22 October 2007
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PubMed ID: 18003335