Abstract:
From an engineering point of view, a kinesin-microtubule biomolecular motor system is an attractive candidate as a driving force in the aqueous environment of micro total...Show MoreMetadata
Abstract:
From an engineering point of view, a kinesin-microtubule biomolecular motor system is an attractive candidate as a driving force in the aqueous environment of micro total analysis systems (/spl mu/TAS). We have studied fundamental components to be integrated in /spl mu/TAS by implementing kinesin-microtubule systems into micro/nanoelectromechanical systems (MEMS/NEMS). This paper presents the cryopreservation technique of microtubules that are immobilized in a microfluidic device. Microtubules work as rail molecules along which kinesin-coated beads are transported; the functionality was preserved even after they were stored at -85/spl deg/C for 30 days. This technique ensures that /spl mu/TAS with immobilized microtubules can be prepared in advance and be thawed for use on demand.
Published in: IEEE Transactions on Advanced Packaging ( Volume: 28, Issue: 4, November 2005)
Department of Micro System Technology, Ritsumeikan University, Shiga, Japan
Ryuji Yokokawa (M'05) received the B.S. and M.S. degrees in mechanical engineering from Kyoto University, Kyoto, Japan, and the Ph.D. degree in electrical engineering from the University of Tokyo, Tokyo, Japan, in 2000, 2002, and 2005, respectively. He also studied MEMS in the Mechanical and Aerospace Engineering Department, University of California, Los Angeles (UCLA), from July 2000 to August 2001.
He is currently an Ass...Show More
Ryuji Yokokawa (M'05) received the B.S. and M.S. degrees in mechanical engineering from Kyoto University, Kyoto, Japan, and the Ph.D. degree in electrical engineering from the University of Tokyo, Tokyo, Japan, in 2000, 2002, and 2005, respectively. He also studied MEMS in the Mechanical and Aerospace Engineering Department, University of California, Los Angeles (UCLA), from July 2000 to August 2001.
He is currently an Ass...View more
Center for International Research on MicroMechatronics, Institute of Industrial Science, University of Tokyo, Tokyo, Japan
Yumi Yoshida received the B.S. and M.S. degrees from the Tokyo University of Science, Tokyo, Japan, in 2001 and 2003, respectively.
She is currently a Research Assistant with the Institute of Industrial Science, University of Tokyo.
Yumi Yoshida received the B.S. and M.S. degrees from the Tokyo University of Science, Tokyo, Japan, in 2001 and 2003, respectively.
She is currently a Research Assistant with the Institute of Industrial Science, University of Tokyo.View more
Center for International Research on MicroMechatronics, Institute of Industrial Science, University of Tokyo, Tokyo, Japan
Shoji Takeuchi (M'03) was born in Japan in 1972. He received the B.E, M.E., and Dr.Eng. degrees in mechanical engineering from the University of Tokyo, Tokyo, Japan, in 1995, 1997, and 2000, respectively.
He is currently an Associate Professor in the Center for International Research on Micromechatronics (CIRMM), Institute of Industrial Science (IIS), University of Tokyo. His current interests include neural interfaces and...Show More
Shoji Takeuchi (M'03) was born in Japan in 1972. He received the B.E, M.E., and Dr.Eng. degrees in mechanical engineering from the University of Tokyo, Tokyo, Japan, in 1995, 1997, and 2000, respectively.
He is currently an Associate Professor in the Center for International Research on Micromechatronics (CIRMM), Institute of Industrial Science (IIS), University of Tokyo. His current interests include neural interfaces and...View more
Center for International Research on MicroMechatronics, Institute of Industrial Science, University of Tokyo, Tokyo, Japan
Takahide Kon received the B.S. and M.S. degrees from the Tokyo Institute of Technology, Tokyo, Japan, and the Ph.D. degree from the University of Tokyo, Tokyo, in 1993, 1995, and 2000, respectively.
He is currently a Research Associate with the University of Tokyo. He is interested in the force-generating mechanism of biological molecular motors including myosin, kinesin, and dynein proteins.
Takahide Kon received the B.S. and M.S. degrees from the Tokyo Institute of Technology, Tokyo, Japan, and the Ph.D. degree from the University of Tokyo, Tokyo, in 1993, 1995, and 2000, respectively.
He is currently a Research Associate with the University of Tokyo. He is interested in the force-generating mechanism of biological molecular motors including myosin, kinesin, and dynein proteins.View more
Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan
Kazuo Sutoh received the Ph.D. degree from the University of Tokyo, Tokyo, Japan, in 1974.
He is currently a Professor in the Department of Life Sciences, University of Tokyo. His current research interest is the molecular mechanism of mechanochemical coupling of motor proteins.
Prof. Sutoh is a Member of the Biophysical Society and the American Society for Cell Biology.
Kazuo Sutoh received the Ph.D. degree from the University of Tokyo, Tokyo, Japan, in 1974.
He is currently a Professor in the Department of Life Sciences, University of Tokyo. His current research interest is the molecular mechanism of mechanochemical coupling of motor proteins.
Prof. Sutoh is a Member of the Biophysical Society and the American Society for Cell Biology.View more
Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan
Hiroyuki Fujita (M'80) received the B.S., M.S., and Ph.D. degrees in electrical engineering from the University of Tokyo, Tokyo, Japan, in 1975, 1977, and 1980, respectively.
Since 2000, he has been a Director of the Center for International Research on MicroMechatronics and Professor (1993 to present), Associate Professor (1981–1993), and Lecturer (1980–1981) with the Institute of Industrial Science, The University of Tok...Show More
Hiroyuki Fujita (M'80) received the B.S., M.S., and Ph.D. degrees in electrical engineering from the University of Tokyo, Tokyo, Japan, in 1975, 1977, and 1980, respectively.
Since 2000, he has been a Director of the Center for International Research on MicroMechatronics and Professor (1993 to present), Associate Professor (1981–1993), and Lecturer (1980–1981) with the Institute of Industrial Science, The University of Tok...View more
Contents Department of Micro System Technology, Ritsumeikan University, Shiga, Japan
Ryuji Yokokawa (M'05) received the B.S. and M.S. degrees in mechanical engineering from Kyoto University, Kyoto, Japan, and the Ph.D. degree in electrical engineering from the University of Tokyo, Tokyo, Japan, in 2000, 2002, and 2005, respectively. He also studied MEMS in the Mechanical and Aerospace Engineering Department, University of California, Los Angeles (UCLA), from July 2000 to August 2001.
He is currently an Assistant Professor with the Department of Micro System Technology, Ritsumeikan University, Shiga, Japan. His current research involves applying biomaterials to micro/nanotechnology and integrating MEMS and motor proteins for a transport system.
Dr. Yokokawa is a member of the IEEJ
Ryuji Yokokawa (M'05) received the B.S. and M.S. degrees in mechanical engineering from Kyoto University, Kyoto, Japan, and the Ph.D. degree in electrical engineering from the University of Tokyo, Tokyo, Japan, in 2000, 2002, and 2005, respectively. He also studied MEMS in the Mechanical and Aerospace Engineering Department, University of California, Los Angeles (UCLA), from July 2000 to August 2001.
He is currently an Assistant Professor with the Department of Micro System Technology, Ritsumeikan University, Shiga, Japan. His current research involves applying biomaterials to micro/nanotechnology and integrating MEMS and motor proteins for a transport system.
Dr. Yokokawa is a member of the IEEJView more
Center for International Research on MicroMechatronics, Institute of Industrial Science, University of Tokyo, Tokyo, Japan
Yumi Yoshida received the B.S. and M.S. degrees from the Tokyo University of Science, Tokyo, Japan, in 2001 and 2003, respectively.
She is currently a Research Assistant with the Institute of Industrial Science, University of Tokyo.
Yumi Yoshida received the B.S. and M.S. degrees from the Tokyo University of Science, Tokyo, Japan, in 2001 and 2003, respectively.
She is currently a Research Assistant with the Institute of Industrial Science, University of Tokyo.View more
Center for International Research on MicroMechatronics, Institute of Industrial Science, University of Tokyo, Tokyo, Japan
Shoji Takeuchi (M'03) was born in Japan in 1972. He received the B.E, M.E., and Dr.Eng. degrees in mechanical engineering from the University of Tokyo, Tokyo, Japan, in 1995, 1997, and 2000, respectively.
He is currently an Associate Professor in the Center for International Research on Micromechatronics (CIRMM), Institute of Industrial Science (IIS), University of Tokyo. His current interests include neural interfaces and bio hybrid MEMS with biomolecular motors.
Prof. Takeuchi received a JSPS research fellowship for young scientists in 2000 and 2001 and the Award for the Advanced Research from the Japan Society of Medical Electronics and Biological Engineering in 2001.
Shoji Takeuchi (M'03) was born in Japan in 1972. He received the B.E, M.E., and Dr.Eng. degrees in mechanical engineering from the University of Tokyo, Tokyo, Japan, in 1995, 1997, and 2000, respectively.
He is currently an Associate Professor in the Center for International Research on Micromechatronics (CIRMM), Institute of Industrial Science (IIS), University of Tokyo. His current interests include neural interfaces and bio hybrid MEMS with biomolecular motors.
Prof. Takeuchi received a JSPS research fellowship for young scientists in 2000 and 2001 and the Award for the Advanced Research from the Japan Society of Medical Electronics and Biological Engineering in 2001.View more
Center for International Research on MicroMechatronics, Institute of Industrial Science, University of Tokyo, Tokyo, Japan
Takahide Kon received the B.S. and M.S. degrees from the Tokyo Institute of Technology, Tokyo, Japan, and the Ph.D. degree from the University of Tokyo, Tokyo, in 1993, 1995, and 2000, respectively.
He is currently a Research Associate with the University of Tokyo. He is interested in the force-generating mechanism of biological molecular motors including myosin, kinesin, and dynein proteins.
Takahide Kon received the B.S. and M.S. degrees from the Tokyo Institute of Technology, Tokyo, Japan, and the Ph.D. degree from the University of Tokyo, Tokyo, in 1993, 1995, and 2000, respectively.
He is currently a Research Associate with the University of Tokyo. He is interested in the force-generating mechanism of biological molecular motors including myosin, kinesin, and dynein proteins.View more
Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan
Kazuo Sutoh received the Ph.D. degree from the University of Tokyo, Tokyo, Japan, in 1974.
He is currently a Professor in the Department of Life Sciences, University of Tokyo. His current research interest is the molecular mechanism of mechanochemical coupling of motor proteins.
Prof. Sutoh is a Member of the Biophysical Society and the American Society for Cell Biology.
Kazuo Sutoh received the Ph.D. degree from the University of Tokyo, Tokyo, Japan, in 1974.
He is currently a Professor in the Department of Life Sciences, University of Tokyo. His current research interest is the molecular mechanism of mechanochemical coupling of motor proteins.
Prof. Sutoh is a Member of the Biophysical Society and the American Society for Cell Biology.View more
Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan
Hiroyuki Fujita (M'80) received the B.S., M.S., and Ph.D. degrees in electrical engineering from the University of Tokyo, Tokyo, Japan, in 1975, 1977, and 1980, respectively.
Since 2000, he has been a Director of the Center for International Research on MicroMechatronics and Professor (1993 to present), Associate Professor (1981–1993), and Lecturer (1980–1981) with the Institute of Industrial Science, The University of Tokyo. He is currently engaged in the investigation of microelectromechanical systems fabricated by IC-based processes and applications to optics, hard disk drives, and bio/nanotechnology. He is also interested in autonomous distributed microsystems.
Dr. Fujita is a member of the IEEJ.
Hiroyuki Fujita (M'80) received the B.S., M.S., and Ph.D. degrees in electrical engineering from the University of Tokyo, Tokyo, Japan, in 1975, 1977, and 1980, respectively.
Since 2000, he has been a Director of the Center for International Research on MicroMechatronics and Professor (1993 to present), Associate Professor (1981–1993), and Lecturer (1980–1981) with the Institute of Industrial Science, The University of Tokyo. He is currently engaged in the investigation of microelectromechanical systems fabricated by IC-based processes and applications to optics, hard disk drives, and bio/nanotechnology. He is also interested in autonomous distributed microsystems.
Dr. Fujita is a member of the IEEJ.View more
