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Evaluation of cryopreserved microtubules immobilized in microfluidic channels for a bead-assay-based transportation system | IEEE Journals & Magazine | IEEE Xplore

Evaluation of cryopreserved microtubules immobilized in microfluidic channels for a bead-assay-based transportation system


Abstract:

From an engineering point of view, a kinesin-microtubule biomolecular motor system is an attractive candidate as a driving force in the aqueous environment of micro total...Show More

Abstract:

From an engineering point of view, a kinesin-microtubule biomolecular motor system is an attractive candidate as a driving force in the aqueous environment of micro total analysis systems (/spl mu/TAS). We have studied fundamental components to be integrated in /spl mu/TAS by implementing kinesin-microtubule systems into micro/nanoelectromechanical systems (MEMS/NEMS). This paper presents the cryopreservation technique of microtubules that are immobilized in a microfluidic device. Microtubules work as rail molecules along which kinesin-coated beads are transported; the functionality was preserved even after they were stored at -85/spl deg/C for 30 days. This technique ensures that /spl mu/TAS with immobilized microtubules can be prepared in advance and be thawed for use on demand.
Published in: IEEE Transactions on Advanced Packaging ( Volume: 28, Issue: 4, November 2005)
Page(s): 577 - 583
Date of Publication: 30 November 2005

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