100% Single Cell Encapsulation via Acoustofluidic Printing Based on a Gigahertz Acoustic Resonator

In this study, an acoustofluidic printing system for generation of single-cell droplets based on a gigahertz acoustic resonator was proposed and verified. The working area of the resonator has a typical dimension of 10×10 micrometer which is very suitable for single cell printing. Single cells were encapsulated in picoliter droplets and printed directly to a flat substrate without any significant influence on their viability. By combining an optic feed-back loop, a 100% single-cell encapsulation rate is achieved.Clinical Relevance— This acoustic-based system has good biocompatibility and high encapsulation rate, which expands the mechanism of medical and biology studies.