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STED microscopy—super-resolution bio-imaging utilizing a stimulated emission depletion | OUP Journals & Magazine | IEEE Xplore

STED microscopy—super-resolution bio-imaging utilizing a stimulated emission depletion

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Abstract:

One of the most popular super-resolution microscopies that breaks the diffraction barrier is stimulated emission depletion (STED) microscopy. As the optical set-up of STE...Show More

Abstract:

One of the most popular super-resolution microscopies that breaks the diffraction barrier is stimulated emission depletion (STED) microscopy. As the optical set-up of STED microscopy is based on a laser scanning microscopy (LSM) system, it potentially has several merits of LSM like confocal or two-photon excitation LSM. In this article, we first describe the principles of STED microscopy and then describe the features of our newly developed two-photon excitation STED microscopy. On the basis of our recent results and those of other researchers, we conclude by discussing future research and new technologies in this field.
Published in: Microscopy ( Volume: 64, Issue: 4, August 2015)
Page(s): 227 - 236
Date of Publication: August 2015

ISSN Information: