1. Introduction
In last couple of decades, advances in microscopy techniques have enabled the investigation of dynamic processes of cells at increasing temporal and spatial resolution. To a large extent, microscopy imaging can be automated resulting in a huge amount of data with single imaging experiments generating up-to TBs of data [1]. Manual analysis of these huge datasets is highly inefficient, not easily reproducible, often only qualitative and limits the hypotheses which can be tested. In cell and developmental biology, to better understand cell functions and tissue development, it is often important to analyze cell behavior at individual cell level. Robust cell segmentation and tracking is necessary for automating these detailed analyses.