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Joint cell segmentation and tracking using cell proposals | IEEE Conference Publication | IEEE Xplore

Joint cell segmentation and tracking using cell proposals


Abstract:

Time-lapse microscopy imaging has advanced rapidly in last few decades and is producing large volume of data in cell and developmental biology. This has increased the imp...Show More

Abstract:

Time-lapse microscopy imaging has advanced rapidly in last few decades and is producing large volume of data in cell and developmental biology. This has increased the importance of automated analyses, which depend heavily on cell segmentation and tracking as these are the initial stages when computing most biologically important cell properties. In this paper, we propose a novel joint cell segmentation and tracking method for fluorescence microscopy sequences, which generates a large set of cell proposals, creates a graph representing different cell events and then iteratively finds the most probable path within this graph providing cell segmentations and tracks. We evaluate our method on three datasets from ISBI Cell Tracking Challenge and show that our greedy nonoptimal joint solution results in improved performance compared with state of the art methods.
Date of Conference: 13-16 April 2016
Date Added to IEEE Xplore: 16 June 2016
Electronic ISBN:978-1-4799-2349-6
Electronic ISSN: 1945-8452
Conference Location: Prague, Czech Republic
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1. Introduction

In last couple of decades, advances in microscopy techniques have enabled the investigation of dynamic processes of cells at increasing temporal and spatial resolution. To a large extent, microscopy imaging can be automated resulting in a huge amount of data with single imaging experiments generating up-to TBs of data [1]. Manual analysis of these huge datasets is highly inefficient, not easily reproducible, often only qualitative and limits the hypotheses which can be tested. In cell and developmental biology, to better understand cell functions and tissue development, it is often important to analyze cell behavior at individual cell level. Robust cell segmentation and tracking is necessary for automating these detailed analyses.

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