Abstract:
A novel, integrated lysis and immunoassay methodology and system for intracellular protein measurement are described. The method uses paramagnetic particles both as a lys...Show MoreMetadata
Abstract:
A novel, integrated lysis and immunoassay methodology and system for intracellular protein measurement are described. The method uses paramagnetic particles both as a lysis agent and assay label resulting in a rapid test requiring minimal operator intervention, the test being homogeneous and completed in less than 10 min. A design study highlights the critical features of the magnetic detection system used to quantify the paramagnetic particles and a novel frequency-locked loop-based magnetometer is presented. A study of paramagnetic particle enhanced lysis demonstrates that the technique is more than twice as efficient at releasing intracellular protein as ultrasonic lysis alone. Results are presented for measurements of intracellular prostate specific antigen in an LNCAP cell line. This model was selected to demonstrate the rapidity and efficiency of intracellular protein quantification. It was shown that, on average, LNCAP cells contained 0.43 fg of prostate specific antigen. This system promises an attractive solution for applications that require a rapid determination of intracellular proteins.
Published in: IEEE Transactions on Biomedical Engineering ( Volume: 60, Issue: 5, May 2013)
Department of Biomedical Science, Qatar University, Doha, Qatar
Elham Sharif received the B.Sc. degree in biomedical sciences with Excellent GPA from Qatar University, Doha, Qatar, and the Ph.D. degree in biomedical sciences (clinical chemistry) from the University of West of England, Bristol, U.K., in January 2009, where her dissertation research was focused on the rapid measurements of LNCAP intracellular PSA as a model for a novel cell-based diagnostic exploiting magneto immunoassa...Show More
Elham Sharif received the B.Sc. degree in biomedical sciences with Excellent GPA from Qatar University, Doha, Qatar, and the Ph.D. degree in biomedical sciences (clinical chemistry) from the University of West of England, Bristol, U.K., in January 2009, where her dissertation research was focused on the rapid measurements of LNCAP intracellular PSA as a model for a novel cell-based diagnostic exploiting magneto immunoassa...View more
Institute of Bio-Sensing Technology, University of the West of England, Bristol, UK
Janice Kiely received a B.Eng. (Hons.) in electronic engineering from the University of Sheffield, Sheffield, U.K. and the Ph.D. degree from the University of Wales, College of Cardiff, Cardiff, U.K., in 1992, where her Ph.D. research involved the development of a novel, miniature thermoelectric device suitable for use as a passive IR detector.
In 1992, she was appointed as a Lecturer specializing in measurement and instru...Show More
Janice Kiely received a B.Eng. (Hons.) in electronic engineering from the University of Sheffield, Sheffield, U.K. and the Ph.D. degree from the University of Wales, College of Cardiff, Cardiff, U.K., in 1992, where her Ph.D. research involved the development of a novel, miniature thermoelectric device suitable for use as a passive IR detector.
In 1992, she was appointed as a Lecturer specializing in measurement and instru...View more
Institute of Bio-Sensing Technology, University of the West of England, Bristol, UK
Patrick Wraith began his engineering career in the Royal Corps of Signals (Army), in Radio Communications (ELINT) in the U.K. and overseas. He moved into Multimedia Engineering and Management posts in the U.K. and with Sony (Pty) in South Africa. He was a Technical Advisor on Digital Satellite Broadcast for Eutelsat, Europes premiere communications satellite organization. He has more than 30 years teaching experience as a...Show More
Patrick Wraith began his engineering career in the Royal Corps of Signals (Army), in Radio Communications (ELINT) in the U.K. and overseas. He moved into Multimedia Engineering and Management posts in the U.K. and with Sony (Pty) in South Africa. He was a Technical Advisor on Digital Satellite Broadcast for Eutelsat, Europes premiere communications satellite organization. He has more than 30 years teaching experience as a...View more
Institute of Bio-Sensing Technology, University of the West of England, Bristol, UK
Richard Luxton received the Ph.D. degree from London University, London, U.K., in 1992.
He was with the National Hospital for Neurology and Neurosurgery, London University, studying the affinity of antibodies synthesized in the brain as a result of infection or multiple sclerosis. He took a post of a Senior Lecturer in clinical biochemistry at the University of the West of England, Bristol, U.K., in 1993 and became a Profe...Show More
Richard Luxton received the Ph.D. degree from London University, London, U.K., in 1992.
He was with the National Hospital for Neurology and Neurosurgery, London University, studying the affinity of antibodies synthesized in the brain as a result of infection or multiple sclerosis. He took a post of a Senior Lecturer in clinical biochemistry at the University of the West of England, Bristol, U.K., in 1993 and became a Profe...View more
Contents Department of Biomedical Science, Qatar University, Doha, Qatar
Elham Sharif received the B.Sc. degree in biomedical sciences with Excellent GPA from Qatar University, Doha, Qatar, and the Ph.D. degree in biomedical sciences (clinical chemistry) from the University of West of England, Bristol, U.K., in January 2009, where her dissertation research was focused on the rapid measurements of LNCAP intracellular PSA as a model for a novel cell-based diagnostic exploiting magneto immunoassay.
In 2001, she was with Qatar University as a Lecturer before leaving for the Ph.D. degree in 2004. She is still with Qatar University as an Assistant Professor of biomedical sciences. She is mainly teaching clinical chemistry, endocrinology, pharmacology, and toxicology. Her research was focused upon using novel methods of utilizing magneto immunoassay. Her current research interest includes conditions related to diseases occurring in Qatar and would like to establish a research group to investigate conditions that affect the life style impact in Qatar especially topics related to womens health. In 2011, she published an article titled: The prevalence of vitamin D deficiency among female college students at Qatar University.
Dr. Elham presented her work several times in international conferences mainly the American Association of Clinical Chemistry. She was awarded the Sigma Diagnostic Prize for Best Overall Achievements in the Master Degree studies.
Elham Sharif received the B.Sc. degree in biomedical sciences with Excellent GPA from Qatar University, Doha, Qatar, and the Ph.D. degree in biomedical sciences (clinical chemistry) from the University of West of England, Bristol, U.K., in January 2009, where her dissertation research was focused on the rapid measurements of LNCAP intracellular PSA as a model for a novel cell-based diagnostic exploiting magneto immunoassay.
In 2001, she was with Qatar University as a Lecturer before leaving for the Ph.D. degree in 2004. She is still with Qatar University as an Assistant Professor of biomedical sciences. She is mainly teaching clinical chemistry, endocrinology, pharmacology, and toxicology. Her research was focused upon using novel methods of utilizing magneto immunoassay. Her current research interest includes conditions related to diseases occurring in Qatar and would like to establish a research group to investigate conditions that affect the life style impact in Qatar especially topics related to womens health. In 2011, she published an article titled: The prevalence of vitamin D deficiency among female college students at Qatar University.
Dr. Elham presented her work several times in international conferences mainly the American Association of Clinical Chemistry. She was awarded the Sigma Diagnostic Prize for Best Overall Achievements in the Master Degree studies.View more
Institute of Bio-Sensing Technology, University of the West of England, Bristol, UK
Janice Kiely received a B.Eng. (Hons.) in electronic engineering from the University of Sheffield, Sheffield, U.K. and the Ph.D. degree from the University of Wales, College of Cardiff, Cardiff, U.K., in 1992, where her Ph.D. research involved the development of a novel, miniature thermoelectric device suitable for use as a passive IR detector.
In 1992, she was appointed as a Lecturer specializing in measurement and instrumentation at the University of the West of England, Bristol, U.K. She has applied this expertise across a number of research fields including power systems and semiconductor device design. Since 1998, her key research interest involves the development of novel biosensing instrumentation, and in 2008, she co-founded the Institute of Bio-sensing Technology, University of the West of England, Bristol, U.K. Currently, as the co-Director of this institute she develops industrial/academic partnership to solve biosensing problems. A core area of her research interest includes the development of immunoassay-based measurement system using paramagnetic particles as labels.
Janice Kiely received a B.Eng. (Hons.) in electronic engineering from the University of Sheffield, Sheffield, U.K. and the Ph.D. degree from the University of Wales, College of Cardiff, Cardiff, U.K., in 1992, where her Ph.D. research involved the development of a novel, miniature thermoelectric device suitable for use as a passive IR detector.
In 1992, she was appointed as a Lecturer specializing in measurement and instrumentation at the University of the West of England, Bristol, U.K. She has applied this expertise across a number of research fields including power systems and semiconductor device design. Since 1998, her key research interest involves the development of novel biosensing instrumentation, and in 2008, she co-founded the Institute of Bio-sensing Technology, University of the West of England, Bristol, U.K. Currently, as the co-Director of this institute she develops industrial/academic partnership to solve biosensing problems. A core area of her research interest includes the development of immunoassay-based measurement system using paramagnetic particles as labels.View more
Institute of Bio-Sensing Technology, University of the West of England, Bristol, UK
Patrick Wraith began his engineering career in the Royal Corps of Signals (Army), in Radio Communications (ELINT) in the U.K. and overseas. He moved into Multimedia Engineering and Management posts in the U.K. and with Sony (Pty) in South Africa. He was a Technical Advisor on Digital Satellite Broadcast for Eutelsat, Europes premiere communications satellite organization. He has more than 30 years teaching experience as a Senior Lecturer/Researcher at the University of the West of England, Bristol, U.K. In addition to teaching Digital and Analogue Electronics subjects, including RF Communications and Embedded Microcomputers, he has been employed in the Research and Development of Magnetic Immuno-Assay (MIA) detection systems, designing and constructing sensitive instruments to detect minute quantities of superparamagnetic particles used in the detection of CKMB, PSA, C. Diff, and others bioentities. This research work, on behalf of the Institute of Bio-Sensing Technology (IBST). IBST is in association with various industrial, medical, and governmental sponsors. He is co-author on several journal papers and is an inventor on a patent filed on the MIA detection of particles in flowing liquids. His research interests, in addition to developing novel biosensor detection systems, include the development of Impedance Spectroscopy Instrumentation.
He was registered I.Eng. and a Fellow of the Institution of Engineering Technology (FIET) in 1990.
Patrick Wraith began his engineering career in the Royal Corps of Signals (Army), in Radio Communications (ELINT) in the U.K. and overseas. He moved into Multimedia Engineering and Management posts in the U.K. and with Sony (Pty) in South Africa. He was a Technical Advisor on Digital Satellite Broadcast for Eutelsat, Europes premiere communications satellite organization. He has more than 30 years teaching experience as a Senior Lecturer/Researcher at the University of the West of England, Bristol, U.K. In addition to teaching Digital and Analogue Electronics subjects, including RF Communications and Embedded Microcomputers, he has been employed in the Research and Development of Magnetic Immuno-Assay (MIA) detection systems, designing and constructing sensitive instruments to detect minute quantities of superparamagnetic particles used in the detection of CKMB, PSA, C. Diff, and others bioentities. This research work, on behalf of the Institute of Bio-Sensing Technology (IBST). IBST is in association with various industrial, medical, and governmental sponsors. He is co-author on several journal papers and is an inventor on a patent filed on the MIA detection of particles in flowing liquids. His research interests, in addition to developing novel biosensor detection systems, include the development of Impedance Spectroscopy Instrumentation.
He was registered I.Eng. and a Fellow of the Institution of Engineering Technology (FIET) in 1990.View more
Institute of Bio-Sensing Technology, University of the West of England, Bristol, UK
Richard Luxton received the Ph.D. degree from London University, London, U.K., in 1992.
He was with the National Hospital for Neurology and Neurosurgery, London University, studying the affinity of antibodies synthesized in the brain as a result of infection or multiple sclerosis. He took a post of a Senior Lecturer in clinical biochemistry at the University of the West of England, Bristol, U.K., in 1993 and became a Professor of biosensing technology in 2009. He is currently the Co-Director of the Institute of Bio-Sensing Technology, University of the West of England, which he co-founded in 2008. His research is focused on the area of developing new rapid detection technologies for point of care diagnostics, environmental analysis, food safety, and homeland defence applications. One area of research that is of great interest to him is the development of whole cell biosensors and in vitro monitoring. In addition, he has been particularly successful in the development of magnetic detection technology for use in immunoassays and he is a named inventor on a number of patents related to this technology.
Dr. Luxton is a Chair of the International Conference for Bio-sensing Technology (Elsevier) and is a Chair of the Board of Directors for MedilinkSW and the Sensors for Water Interest Group, both trade associations promoting technology. He launched the South West Biomedical Innovation Network which supports the commercialization of innovation in small and medium enterprises in the South West of England. An important aspect of this work is to establish a Centre to develop new cell monitoring technology.
Richard Luxton received the Ph.D. degree from London University, London, U.K., in 1992.
He was with the National Hospital for Neurology and Neurosurgery, London University, studying the affinity of antibodies synthesized in the brain as a result of infection or multiple sclerosis. He took a post of a Senior Lecturer in clinical biochemistry at the University of the West of England, Bristol, U.K., in 1993 and became a Professor of biosensing technology in 2009. He is currently the Co-Director of the Institute of Bio-Sensing Technology, University of the West of England, which he co-founded in 2008. His research is focused on the area of developing new rapid detection technologies for point of care diagnostics, environmental analysis, food safety, and homeland defence applications. One area of research that is of great interest to him is the development of whole cell biosensors and in vitro monitoring. In addition, he has been particularly successful in the development of magnetic detection technology for use in immunoassays and he is a named inventor on a number of patents related to this technology.
Dr. Luxton is a Chair of the International Conference for Bio-sensing Technology (Elsevier) and is a Chair of the Board of Directors for MedilinkSW and the Sensors for Water Interest Group, both trade associations promoting technology. He launched the South West Biomedical Innovation Network which supports the commercialization of innovation in small and medium enterprises in the South West of England. An important aspect of this work is to establish a Centre to develop new cell monitoring technology.View more
