Efficient and high-throughput electroporation chips

To fulfill demands of high efficient in vitro and in vivo nucleic acid delivery, three types of electroporation microchips were developed. To better fit the standard multi-well plate, we designed a chip with annual interdigital electrodes which generate even electric field. High efficient DNA and siRNA delivery was achieved on both supernatant and adherent cells. The parylene based substrate was employed to realize in vivo electroporation. The flexible film fits the natural shape of the electroporated objects and has good biocompatibility, which makes the chip potential for implantable electroporation devices. Besides, to meet requirements of continuous cell treatment, we designed a flow-type electroporation chip, in which the sheath flow was employed to isolate cells from harmful effects due to water electrolysis around electrodes.