Detecting modes in fed-batch fermented process. Application to Escherichia Coli Cultivation | IEEE Conference Publication | IEEE Xplore

Detecting modes in fed-batch fermented process. Application to Escherichia Coli Cultivation


Abstract:

This paper presents a strategy based on residual generation to detect acetate formation during a culture of Escherichia coli as producer of recombinant proteins. Indeed p...Show More

Abstract:

This paper presents a strategy based on residual generation to detect acetate formation during a culture of Escherichia coli as producer of recombinant proteins. Indeed protein productivity is both affected by acetate concentration that inhibits cell growth and by acetate production that reduces protein production. Formation of acetate occurs when the specific glucose uptake exceeds a critical value. This situation must consequently be avoided. The proposed approach deeply uses a model that gathers the knowledge about the process. It aims to study the possibility to identify the current biological behaviour from this model and from a reduced instrumentation. Depending on the instrumentation, specialized residuals are generated for the different biological modes. These residuals, calculated from the available measurement, have to be close to zero when the mode they have been design for is active. According to the residuals that trigger a deviation from zero test, it is then possible to recognize the current mode and, that way, to determine the strain biological activity
Date of Conference: 04-06 October 2006
Date Added to IEEE Xplore: 30 July 2007
ISBN Information:
Conference Location: Beijing, China

I. Introduction

Monitoring fermentation processes has been address for a long time. First, measurements errors were concerned and data reconciliation techniques from stoechiometric considerations were used [WAN,83]; [VAN,94]). Another approach utilized data miming or data analysis to deeper exploit information that is contained in repeated fed-batch cultures ([STE, 97]; [KAM, 00]; [LEN, 01]; [NOM, 95]). The origin of the deviation between expected behavior and the actual was then difficult to establish.

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References

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