Abstract:
A PDMS microfluidic device composed of several microstructured layers is fabricated for perfusion culture of mammalian cells. The device has been tested with Hep G2 and 3...Show MoreMetadata
Abstract:
A PDMS microfluidic device composed of several microstructured layers is fabricated for perfusion culture of mammalian cells. The device has been tested with Hep G2 and 3T3-L1 cells to investigate the possibilities of microfluidic/MEMS devices in the field of tissue engineering. Comparison between a two layer device and multi-layer devices with and without oxygen chamber has shown the importance of oxygen supply in large scale cell culture required for tissue reconstruction.
Published in: The Sixteenth Annual International Conference on Micro Electro Mechanical Systems, 2003. MEMS-03 Kyoto. IEEE
Date of Conference: 23-23 January 2003
Date Added to IEEE Xplore: 02 April 2003
Print ISBN:0-7803-7744-3
Print ISSN: 1084-6999