MSRC Measurements of High Frequency Non Ionizing Electromagnetic Radiations (NIR) on Living Organisms

The aim of this paper is to demonstrate responses in living organisms to electromagnetic radiation fields. This is æhieved by a molecular approach which is based on (i) a very accurate method to measure the stress- related transcripts (Real-Time Quantitative-PCR), (ii) the rapidity of the method which allows avoiding all types of external contamination. (iii) plants as a biological model: they are immobile organism that can’t escape the stimulation, and therefore are extremely sensitive to environmental stimulations. The stimulation (5V/m, 900MHz) is given to tomato plants (Lycopersicon esculentum, Solanaceae) using a Mode Stirred Reverberation Chamber. In plants grown under light/dark alternance, a rapid (5-15 min), transient and important (2-5 fold) increase in the accumulation of the LebZIP1 transcription factor transcripts is measured. In contrast, when plants are shielded, no modification in transcript accumulation is observed. Discussions about these results are provided and perspectives are suggested.