Loading [MathJax]/extensions/TeX/mhchem.js
Indirect sandwich aptamer assay coupled structurally stabilized hairpin assisted hybridization chain reaction for femtomolar detection of cardiac troponin I | IEEE Conference Publication | IEEE Xplore

Indirect sandwich aptamer assay coupled structurally stabilized hairpin assisted hybridization chain reaction for femtomolar detection of cardiac troponin I

Publisher: IEEE

Abstract:

In this study, we present the development of a rapid, sensitive, and precise method for the detection of cardiac troponin I, which is crucial for early identification and...View more

Abstract:

In this study, we present the development of a rapid, sensitive, and precise method for the detection of cardiac troponin I, which is crucial for early identification and intervention in acute myocardial infarction, particularly in resource-limited settings. Our approach employs a colorimetric, ultrasensitive indirect sandwich aptamer assay for cardiac troponin I detection. To enhance the structural stability of hairpins and prevent nonspecific amplification, we incorporated trimeric DNAzyme sequences required for the generation of color into the hairpin loop regions. This novel technique has the potential to significantly improve diagnostic capabilities for acute myocardial infarction in resource-constrained areas.
Date of Conference: 30 July 2023 - 01 August 2023
Date Added to IEEE Xplore: 17 October 2023
ISBN Information:
Publisher: IEEE
Conference Location: London, United Kingdom

Funding Agency:


References

References is not available for this document.