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Entertainment and multimedia are the key functionalities in emerging mobile markets. The ability to understand and quantify the quality of experience (QoE, i.e., the users' subjective perception of the “overall acceptability of an application or service”), will play a major role in the success of these mobile services. This study explores the thresholds at which the technical quality of a mobile video service becomes unacceptable for users. A subjective experiment drawing on the logging of technical parameters combined with subjective evaluations by a user panel resulted in a model for quantifying the acceptability of video interruptions. The results of this analysis provide insights into the QoE and (un)acceptability regarding video interruptions for different network conditions and video parameters. The conclusions of this paper can be used as a guideline for service design and network dimensioning.