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Rigorous mathematical method of moments (MoM) for analyzing spherical microstrip aperture-coupled antennas is presented in this paper by using dyadic Green's functions (DGF) in conjunction with magnetic field integral equation (MFIE) and electric field integral equation (EFIE) formulations. With the aid of linear Rao-Wilton-Glisson (RWG) triangular basis functions, spherical three-layer aperture-coupled patch antennas are analyzed. The effect of mutual coupling between slot and patch antenna is developed and the input impedance and radiation patterns of such an antenna are presented. A prototype of a spherical aperture-coupled antenna is fabricated and tested. The measurement results are compared to the results of the proposed method and a good agreement between the results is achieved. The measured efficiency and gain of this antenna at 3.15 GHz are 85% and 5.23 dB, respectively.