Increase of Fibroblast Proliferation by Composite Membrane (Polyvinyl Alcohol - Collagen - Hydroxyapatite)

The wound healing process depends on the presence of high fibroblast numbers. The use of a composite membrane is known to increase its proliferation. This study evaluated the effect of a composite membrane (Polyvinyl Alcohol Collagen Hydroxyapatite) derived from fish scales (Lates calcarifer) in vitro. Fibroblast proliferation test was performed using 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) assay. Cultured fibroblasts treated with the irradiated composite membrane (15kGy and 25kGy) compared with the untreated group as a control. The observation was done at 24, 48, and 72 hours. Tukey’s test showed no significant difference between the all irradiated groups (P 0.449), while there was a significant difference between the 24 and 48 hours group (P 0.049), as well as the 24 and 72 hours group (P 0.000). The experiment showed the average fibroblast proliferation increase in the irradiated groups compared to the control group; the average was 346.09% in 15kGy and 373.70% in 25kGy. The composite membrane (PVA Collagen HA) could increase the fibroblast growth rate of proliferation in vitro since 24 hours observation compared to the control group, and the highest growth rate was 13.58 times in 15kGy for 24 hours at 48 hours. Composite membrane indicating the potential use for Guided Tissue Regeneration (GTR).