Analysis of Ehrlich ascites carcinoma with electron paramagnetic resonance

The study was aimed to reveal the dependence of changes in the content of metal-containing proteins and freeradical forms in a suspension of the experimental tumor in different periods of tumor growth using electron paramagnetic resonance. The object of the study was Ehrlich's ascites carcinoma, an experimental undifferentiated tumor, which is used to study various aspects of tumor development under external influences. Ehrlich ascites carcinoma was inoculated into the peritoneal cavity of laboratory mice. We believe that the determination of the concentrations of metal-containing proteins and free-radical forms using this method may be an important prognostic parameter for assessing the state of the tumor. Changes in metabolism will inevitably affect the functionality of cells and, as a consequence, the dynamics of tumor growth. The results of the study showed that metalcontaining proteins belonging to ascites plasma, such as transferrin, ferritin and ceruloplasmin, do not undergo significant changes. The signal from molybdenum-containing proteins also does not change at the stages of experimental tumor growth under consideration. However, in the course of this experiment, it was possible to record a slight decrease in the intensity of the formation of denitrosyl iron complexes with the development of a tumor in the body.