Dual-axes confocal microscopy for clinical skin imaging

We demonstrate MEMS based near infrared fluorescence dual-axes confocal (DAC) imaging in a 10-mm-diameter microscope for three-dimensional (3-D) imaging of in vivo human skin. The probe is used to reveal mosaicing two-dimensional (2-D) en face sub-surface histology at depths of up to 250 mum. The probe has a maximum field of view (FOV) of 800times450 mum
²
and a maximum acquisition rate of 10 frames/second. The transverse and axial resolutions are 5 mum and 7 mum, respectively.