Comparative proteomic analysis of neural stem cells between differentiating and undifferentiating to dopaminergic neuron

The neural stem cells were induced by low-level dopamine (DA). We allowed cells 72h of differentiation before protein extraction. The global proteins of neural stem cells and dopaminergic neurons were extracted. In this study, the protein from cell lysat was rudiment separated by SDS-PAGE, and the gel strip was cut into several portions. The protein in each portion is digested by trypsin, and then the peptides mixtures were analyzed by mass spectrometry (LC-MS/MS). 839 proteins in the neural stem cells and 718 proteins in the dopaminergic neuron were identified. The nestin protein was found in the NSCs and the Sodium-dependent dopamine transporter (DA transporter)(DAT) was found in the dopaminergic neuron which was induced by low level DA. The cytoskeletal proteins rearranged after the NSCs differentiated and regulating proteins changed a lot.