Real-time Detection of Protein Interactions Using Pyrolyzed Carbon

We present label-free, real-time detection of streptavidin binding using the pyrolyzed carbon with a p-type conduction behavior in aqueous solution, which is functionalized with amine-conjugated biotin. Pyrolyzed carbon derived from AZ 5214E are fabricated by photolithography and photoresist thermal decomposition in a N2 atmosphere. The streptavidin-biotin interactions on the pyrolyzed carbon are detected successfully by current change after streptavidin binding.