Variation of Physical Parameters and Anomalous Healing Observation in Plasma Wound Healing

The plasma healing of acute wound on 56 hairless mice is examined and initiated by several externally controlled physical parameters such as driving frequency and power, duration and interval of plasma treatment, a fraction of air mixture, the gap distance between plasma and wound, and others. The variation of these physical parameters results in a different amount of reactive oxygen and nitrogen species (RONS), which stimulate the regulation of RONS in mouse tissue before revealing several important bioindicators in the process of wound healing. While focusing on an important physical indicator such as the wound size, the limited examination with 56 mice showed promising results [much improved over the previous mice experiments by single-frequency plasma (SP)] by Dual-frequency plasma (DP) with 2% air mixture to argon, which effectively correlates with the observation of optical emission spectra. Circular acute wounds treated by DP (2%) were reduced to 24% in just 8 h after the treatment and further reduced to 5% within a few days.