Impact of injection time on migration of SPECT seizure onset in temporal lobe epilepsy

In this work, we investigated an impact of injection time on migration of seizure-onset in ictal/interictal single photon emission computed tomography (SPECT) study for patients with temporal lobe epilepsy. We selected 33 patients with refractory temporal lobe epilepsy who underwent ictal/interictal SPECT studies and had preoperative intracranial EEG result or surgical resection which was used as reference for seizure location. We divided all patients into two groups, which are the fast and the delayed groups; the delayed group comprised patients with injection time more than a cutoff time and vice versa. Using the subtraction ictal-interictal SPECT co-registered with SPECT (SISCOS) with varied Z-threshold (1.0, 1.5, 2.0 and 2.5), a method similar to subtraction ictal SPECT co-registered to magnetic resonance imaging (MRI) (SISCOM), a seizure-onset region in the SISCOS image was localized at the region with maximum sum of Z-scores. For each pair of cutoff time and Z-threshold, we determined the migratory proportion which was defined as the proportion of patients whose seizure-onset location based on SISCOS image was discordant with the reference. At cutoff time of 32-35 seconds and the Z-threshold of 2.0, the migratory proportion values were 7/26 (26.9%) and 5/7 (71.4%) in the fast and the delayed groups, respectively. At the same range of cutoff time with the Z-threshold of 2.5, the migratory proportion was 8/26 (30.8%) in the fast group while the proportion was 5/7 (71.4%) in the delayed group. Using Fisher's exact test, the migratory proportion values at the Z-threshold of 2.0 and 2.5 were significantly different between the fast and the delayed groups (p = 0.0709 and 0.0838, respectively), suggesting that patients with temporal lobe epilepsy who undergo an ictal/interictal SPECT study with injection time longer than 35 seconds tend to have seizure-onset zone migration in the SISCOS analysis with the traditionally-used Z-threshold of 2.0.