Time-resolved Two-photon Excitation Fluorescence Spectroscopy Using a Streak Camera

We present a time-resolved two-photon excitation fluorescence spectroscopy system that is based on a picosecond streak camera and a prism spectrophotometer, and can perform simultaneous fluorescence spectrum and lifetime measurement using two-photon excitation. The potential applications of the system in biomedicine have been demonstrated with tissue endogenous fluorophore (flavin adenine dinucleotide), plant specimen (aloe epidermis) and gastric smooth muscle tissue.