Role of Spin on Future Biomedical Science: Logical Manipulation of Living Cells for Novel Cells-On-Chip

The manipulation ability of individual cells has significant applications for gene sequencing, protein and bio-content analysis in single cell level for their heterogeneity related with drug resistance aiming to lab-on-organ and new drug development. Even though, various single cell platforms are exit, it is still challenge and complex to collect rare cells and their digital manipulation in large-scale operation. In recent years the flexibility of magnetic transport technology using nano/micro scale magnets for the digital magnetophoresis has experienced tremendous advances and has been used for a wide variety of single cells manipulation tasks such as selection, capture, transport, encapsulation, transfection, or lysing of magnetically labeled and un-labeled cells. The magnetic transport technology, which can be integrated within microfluidic channels, relies on both magnetic energy and force tunability and remote control implemented by micro-and nano-patterned magnetic structures. Here, we demonstrate a class of integrated magnetic track circuits for executing sequential and parallel, timed operations on an ensemble of single particles and cells. The magnetic circuitry tracks are designed by conventional lift-off technology and were used for the passive control of cells/particles similar concept to electrical conductor, diodes and capacitor. When the magnetic tracks are combined into arrays and driven by rotating magnetic field, the single cells are precisely control for multiplexed analysis. In addition, the concentric cell translocation and separation were performed by the assembly of this magnetic track into a novel architecture, resembled with spider web network, where all the cells are concentrated into one position and then transported to apartments array for the single cell analysis.