Automated Evaluation of Sperm Membrane Structural Integrity using Open Source Software

Assisted reproductive techniques like, In Vitro Fertilization (IVF), Intra Cytoplasmic Sperm Injection (ICSI) and Intracytoplasmic morphologically-selected sperm injection (IMSI). Live sperm is necessary to maximize the chances of fertilization. Using sperm vitality analysis we can achieve fertilization with very few spermatozoa. By combining good sperm preparation techniques with sperm vitality analysis, it is possible to optimize the results, as well as number of opportunities for conception. In this paper we developed a MACRO on IMAGEJ platform which will identify live and dead sperms. And results are compared with visual method.