The Changes of Submaxilary Glands Trefoil Factor2 Gene and Trefoil Factor3 Gene during Experimental Gastric Ulcer in Rat

To explore the expression changes of trefoil factor 2 (TFF2) and trefoil factor3 (TFF3) gene in submandibular glands during the self healing of experimental gastric ulcer in rats for revealing the relationship between them. The expression of TFF2, TFF3 peptide and genes were detected by immunohistochemistry and RT-PCR methods respectively in gastric ulcer group ,saline control group and normal group. The results were as follows: (1) TFF2 peptide showed strong expression in dark cells with few granules or in those cells without granules, but weakly positive or negative in cells with many granules. The expression was stronger in male rats than that in female rats. The optical density values (OD) values obviously increased on the day 1 after gastric ulcer (P<;0.01), and the lowest on the day 2, then gradually increased again on the day 4, 6 (P<;0.01) , and kept on a high level till 10d to 23d (P<;0.05). Compared with normal group, the OD of TFF2/GAPDH in 1,4,6,10,14 and 23d in gastric ulcer group were obviously higher than that in normal group except the 2d (P<;0.01 or P<;0.05). (2) TFF3 expression was higher in gastric ulcer than that in normal groups on the first day after surgery (P<;0.01), the optical density values (OD) were lower on the 2nd day than that on the 1st day (P<;0.05), then increased gradually on the 4th day and 6th day after gastric ulcer, and reached the highest on the 10th day (P<;0.01), It was still higher on the 23rd than that in normal group (P<;0.05). The OD values of TFF3/GAPDH in gastric ulcer group on the day of 1, 2, 4, 6, 10, 14, 23 are obviously higher than that in normal group (P<;0.01). Rat submaxillary glands TFF2 and TFF3 gene is chiefly in submaxillary ducts of normal and gastric ulcer rat.They may participate in the regulation of self-healing in gastric ulcer with the maner of exocrine.