Intracavity Second Harmonic Generation of Rapid and Random Wavelength Tuned Picosecond Pulsed Laser and its Biological Applications

In this work presents a picosecond pulsed Ti:sapphire laser pumped with a frequency-doubled LD-pumped cw Nd:YVO
₄
laser and intracavity second harmonic generation (SHG) of picosecond pulsed rapid and random wavelength tuned Tksapphire laser. The laser allows the tuning range from UV to blue with picosecond duration. Moreover, the paper demonstrated a pumping of fluorescent proteins (FPs) using the barm labeled cyan fluorescent protein (CFP) as a sample for the floresence microscope.