Supplement of iron abrogates SARS-CoV-2 pseudovirus infection in a 3D model of vascularized organoids

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cause severe outbreak of coronavirus disease 2019 (COVID-19). Even though vaccination, the spread of SARS-CoV-2 is still continue. It is urgent to have a model that can efficiently evaluate potential therapeutic agents to counteract SARS-CoV-2 infection. Iron is an essential molecule for maintaining homeostasis. Supplement of iron significantly to affect virus infection. But the detailed mechanisms of iron on regulating SARS-CoV-2 infection are still unveiled. The three-dimensional (3D) model is a promising system for drug screening and disease progression analysis. Organoid is a typical 3D culture system that recapitulates genetic characteristics and phenotypic features of organs within body. Vasculature is prevalent for all various organs or tumors in the body which transport nutrients, oxygen and metabolites to maintain cellular homeostasis. Thus, we have established a 3D model of vascularized organoid to evaluate the effects of iron on infectivity of SARS-CoV-2 pseudovirus to provide the novel therapeutic strategy in coping SARS-CoV-2 infection.