Handheld fluorometer for detection of blue/green fluorescence

A handheld fluorometer for detecting blue/green fluorescence from small Stokes shift fluorophores is presented in this paper. Two novel techniques have been introduced to emulate far-field operation while operating in the near field. A sensitivity of 26 (seconds per decade of dilution) and a resolution of 0.01 (concentration units) was achieved for detection of Alexa Fluor 488.