Detection of Diarrhoeagenic Escherichia Coli Among Other Bacterial Species Isolated from Children Patients by Automated Methods and Culture based Techniques

Diarrhea is one of the most widespread medical problems, and it can be caused by a variety of microorganisms, including parasites, viruses, and bacteria. Intestinal bacteria (Diarrheagenic E.coli) is one of the most important, since infection with it results in hospitalization and, in some cases, death, particularly in youngsters. The goal of the investigation was to see if (Diarrheagenic E.coli) was spreading in Thi Qar after it was isolated from children with diarrheal infections. From Bint Al-Huda Maternity and Children Hospitals and Mohammed Al-Mousawi Hospital for Children, 430 diarrhea samples were obtained from children under the age of five, both gender, as well as 40 samples from healthy children as control samples. Laboratory and molecular techniques were used to diagnose all of the isolates. Statistical analysis of the samples indicated an infection rate of 180 (41.86%) with bacteria, while other microbial causes showed an infection rate of (58,14%). Escherichia coli was prevalent with a percentage of 180/100 (55,56 %). In this study, we used control strains to detect DEC in pure cultures and spiked stool samples. DEC has a detection limit using multiplex PCR methods. In comparison to conventional lab procedures, we may consider PCR to be a very sensitive and specific molecular biology approach for the identification of virulence gene in different clinical specimens based on this and other research.