Ascorbic Acid Detection Using Spectrometer and Deuterium and Halogen Light Source

The goal of this study is to determine the ascorbic acid (AA) refractive index using a digital refractometer and absorbance spectrum using Ultraviolet-Near Infrared (UV-NIR) spectrometer. The absorption technique was proposed to detect different ascorbic acid concentrations and their absorption wavelength. The mechanism of ascorbic acid detection is based on energy absorbed by the ascorbic acid solution. The results show that the refractive index of ascorbic acid solution from 0.2M to 1.0 M increases with the ascorbic acid concentration and correlates linearly. The absorbance spectra of ascorbic acid solution increase linearly with an ascorbic acid concentration in the 300.69 nm wavelength.