Enzymatic Boolean Logic Gates toward ON/OFF Sensing

In this paper we report a combined theoretical and experimental approach to develop enzyme logic gates networks (ELGN). ELGNs were tested in solution with optical analysis of the output signals. Then, the enzymes were immobilized on a buckypaper electrode for electrochemical transduction of the output signals. Finally, the switchable electrodes were integrated in a biofuel cell as a self-powered device for the detection of sucrose in adulterated wine samples.