Application of different approaches to increase the sensitivity of electrochemiluminescence biosensor for microRNA detection

The detection of microRNA is growing a hotpot using electrochemiluminescence (ECL) biosensors. The mechanism of ECL biosensors can be divided in three parts, which is ion annihilation process, coreactant process and hot electron-induced cathodic process. Based on such mechanism, the approaches to increase the sensitivity of ECL biosensors, including catalyzed hairpin assembly, hybridization chain reaction and nitrogen doped carbon quantum dots has been introduced. ECL-based biosensing is a novel detection method, allowing it to present broad applications in diverse fields of biochemistry.