An Alternative Approach for Bacterial Growth Control: Pseudomonas spp. Imprinted Polymer-Based Surface Plasmon Resonance Sensor

Here,
Pseudomonas
spp. molecularly imprinted polymer-based surface plasmon resonance (MIP-based SPR) sensor has been prepared to perform quantitative analysis of
Pseudomonas
spp simultaneously. The polymeric nanofilm is imprinted on the SPR sensor surface with the microcontact printing method. Nanofilm was characterized with contact angle Non-Imprinted Polymer (NIP) and MIP SPR chips were found at 68.7° and 79.5°, respectively), ellipsometer measurement (the thickness of MIP and NIP nanofilm determined 150.4 ± 1.5 nm and 72.4 ± 1.2 nm values, respectively) and SEM analysis. The different
Pseudomonas
spp. were prepared at a concentration range of
$1 \times 10^{2}$
-
$1\,\,\times10$
⁴
CFU/mL and passed through the SPR system. The total detection time for all steps was reported in 150 seconds for microorganism detection, which is very short compared to the cultural method (2 days). The detection of limit value was calculated
$0.5\,\,\times10$
²
CFU/mL. Selectivity of
Pseudomonas
spp. nanofilm based SPR sensors %
$\boldsymbol {\Delta } \text{R}$
value was determined 8.3 for MIP and 0.25 for NIP. The relative selectivity coefficients (
$\text{k}^\prime $
) of MIP nanofilm based SPR sensor for
Staphylococcus aureus
,
Salmonella paratyphi
and
Escherichia coli
were reported 25.82, 31.69 and 31.24 times, respectively. According to the data obtained in this study, the prepared
Pseudomonas
spp, suppressed surface plasmon resonance sensor, can be used as an alternative to the current cultural method and effective suppression quality, selectivity, and affinity.