Effects Of Laminin Derived Peptides On HBMSC-HUVEC Coculture

In this study, the effects of laminin-derived peptides on hBMSC-HUVEC Scaffold Free Microtissues (SFMs) were investigated. For this purpose, IKVAV, GRGDS and YIGSR peptides were synthesized in solid phase. Microwells were created by using agarose. SFMs were incubated with 0.5 mM, 1 mM and 2 mM laminin-derived peptides media. Experimental groups were compared with negative control which doesnt include any serum and peptide. On the 1st, 4th and 7th days, images were taken under the microscope and diameter analyzes were performed. Image-J program was used for dimensional analysis. Cell Viability Assay was used for viability analysis of SFMs. In terms of the viability and diameter analysis, the highest values of laminin-derived peptides were observed as 0.5 mM YIGSR, 1 mM IKVAV, and 2 mM GRGDS groups.