Evaluation Of ATP Production Of Low Level Laser Therapy In Monolayer And 3 Dimensional Cell Culture

Low level laser therapy (LLLT) is a photo therapy which is used in bone damages. LLLT increases cell proliferation, stimulates osteogenesis and induces bone healing. Main mechanism of osteogenic differentiation via LLLT may depend on ATP level. Effects of 655 nm and 808 nm lasers were evaluated by using wound healing process in vitro monolayer system. Moreover, LLLT based ATP productions were evaluated. ATP levels of 655 nm and 808 nm lasers with 1J/cm2, 3J/cm2 and 5 J/cm2 energy dosages were compared with monolayer and 3D SFMs. Therefore, the study aimed that ATP level comparison between monolayer and SFMs. It contributed to enlighten ATP mechanisms of LLLT during HUVEC:hBMSC co-culture.