Lycorine Nanoparticles Induce Apoptosis Through Mitochondrial Intrinsic Pathway and Inhibit Migration and Invasion in HepG2 Cells

Lycorine-nanoparticles (LYC-NPs) were successfully synthesized using anti-solvent precipitation-freeze drying method, and characterized using transmission electron microscopy (TEM), particle size analysis and Fourier transform infrared spectroscopy (FTIR). Then, the antitumor effects of LYC-NPs against HepG2 cells were investigated, and the underlying molecular mechanisms were explored. Our results showed that LYC-NPs displayed potent antiproliferative against HepG2 cells concentration dependently. Flow cytometry analysis exhibited that LYC-NPs triggered apoptosis and impeded cell cycle in G0/G1 phase. Moreover, the up-regulated expression of cleaved caspases-3 and Bax, and decrease of mitochondrial membrane potential and the Bcl-2 expression were involved in LYC-NPs apoptosis, implying that LYC-NPs induced apoptosis via the mitochondrial-mediated apoptosis pathway. Furthermore, LYC-NPs distinctly impaired HepG2 cells migration and invasion with down-regulation of matrix metalloproteinase-2 (MMP-2) and MMP-9 expression. These results indicated that LYC-NPs could be an favorable agent for restraining the growth and metastasis of HepG2 cells.