Analysis of Mosquito Genetic with PCR-RAPD Approach

The wide distribution of mosquitoes in the territory of Indonesia with differences in geographical location causes mosquitoes to have the ability to adapt to the environment. The ability to adapt to the environment results in high variations phenotypically and genotypically so that Mutation, recombination or migration of genes is the cause of genetic diversity in a population. The purpose of this research is to study the characteristics and inventory of distribution areas, as well as to analyze genetic variations using morphological and molecular approaches. Genetic typology data through the RAPD approach provides information about the genetic variation of mosquitoes in an effort to control them as disease vectors. Genetic characterization was carried out by PCR-RAPD using three primers, namely OPA 11, OPA 12, OPA 15 and OPL12. Data were analyzed using UPGMA with a similarity coefficient using the Jaccard Coefficient of Similarity presented as a dendrogram. The results showed that the variation of the mosquito polymorphy band from the Kelurahan Kemelak Bindung Langit, Komering Ulu Regency was high. With the DNA band profile of RAPD results as many as 37 bands with a polymorphy DNA band presentation of (86.65%).