Effect of Moringa oleifera Leaves on Human Blood Coagulation Process

Local hemostatic agent is required after tooth extraction to accelerate blood clotting in the wound healing process. There are various commercial hemostatic agents on the market; the raw material is quite expensive. Moringa oleifera leaves are known to accelerate the wound healing process. Protease in M. oleifera functions as a coagulant in wastewater treatment and food industry, research on protease as a blood coagulant is still limited. This study aims to examine the effect of M. oleifera leaves extract in the blood coagulation process. The experiments assessed into five groups: positive control, negative control, M. oleifera leaves extract at concentrations of 25%, 50%, and 100%. Prothrombin Time (PT) test used to measure blood coagulation duration of human blood plasma. M. oleifera leaves extract can accelerate the human blood coagulation process compared to the positive control; 1.64 times shorter in 100% concentrate, 1.27 times shorter in 50% concentrate, and 1.08 times shorter in 25% concentrate. The shortest blood coagulation time occurred in M. oleifera leaves extract at 100% concentration with the blood coagulation time was 189.50 ± 19.77 seconds; 1.64 times shorter than the positive control, which was 310.50 ± 28.3 seconds; indicating its potential use for local hemostatic agent.