Quantification Of The Interaction Of Hemosorbents With Red Blood Cells Using Scanning Flow Cytometry

Extracorporeal blood purification by extracting circulating toxins during perfusion through columns filled with a sorbent (hemosorption) is an effective, technically simple and relatively safe method that allows you to quickly solve the problems of detoxification, correction of metabolic disorders of the body as a whole. At the same time, it is important to assess the safety of solid porous sorbents in relation to the biological tissues of the body; primarily, red blood cells. The carbon-free hydrophilic-hydrophobic sorbent γ-Al2 O3@PDMS was studied with this goal in mind, obtained by immobilizing the organosilicon polymer polydimethylsiloxane (PDMS) on the surface of hydrophilic mineral matrix - gamma aluminum oxide a large pore volume and a high specific surface area (about 200 m2/g), determined by the BET method. The sorption activity determined spectrophotometrically by the methylene blue dye is 10 mg/g of the sorbent. The sorption of staphylococcus according to the standard method is 38% The results were compared with a medical carbon-mineral hemosorbent (SUMS-I) obtained using the same mineral matrix - gamma aluminum oxide The results were compared with a medical carbon-mineral hemosorbent (SUMS-I) obtained using the same mineral matrix - gamma aluminum oxide. Data were obtained on the effect of sorbents on the functional parameters of red blood ells, including the rate of anion exchange and the ultimate extensibility of membranes. The study revealed almost no traumatic effect of sorbents on red blood cells.