Modeling Early Indicators of Grapevine Physiology Using Hyperspectral Imaging and Partial Least Squares Regression (PLSR)

In this contribution, we use field-based hyperspectral imaging (HSI) and partial least squares regression (PLSR) to estimate early indicators of grapevine physiological indicators, and analyze identified significant spectral regions for fast and accurate plant health monitoring. HSI and physiological measurements were carried out at two commercial vineyards in California, USA. The PLSR models were developed between reflectance spectra extracted from hyperspectral images and four vine physiological parameters, including stomatal conductance (G
_s
) photosynthetic CO
₂
rate (A), intercellular CO
₂
concentration (Ci) and transpiration rate (E). The results demonstrate PLSR models to predict physiological parameters ( R
²
≥ 0.6), and the best model was found for Gs (R
²
=0.7). The identified significant spectral regions overlap with most commonly used remote sensing stress indicator, suggesting that HSI coupled with PLSR has great potential for upscaling and broader agricultural applications.