Determination of Optimum Concentration of NGR Peptide With Anticancer Effect On Breast Cancer Microtissue

Breast cancer is a fatal disease, and it is one of the most common cancer types among women in the world. The traditional monolayer methods are used to treat diseases. However, the method is limited in terms of the cell to cell communication and responses of cells to drugs. One of the main goals of cancer treatments is to prevent tumor metastasis and prevent diffusion in various areas of the body, thereby it is needed to increase the effectiveness of the treatments and reduce side effects. Peptides can be used in cancer treatment. Most peptide studies are performed in monolayer culture. These cultures can not accurately represent the complex intercellular and intracellular environment in clinical studies. Peptide studies must be performed in scaffold-free conditions to mimic the natural responses of cells. The study has been performed as scaffold-free microtissues with different NGR peptide concentrations. Results have been evaluated in terms of diameters, and viability of microtissues. It is concluded that 2 mM NGR is the most effective concentration in MCF-7 microtissue treatment.