A Correlation Between Electric Fields That Target the Cell Membrane Potential and Dividing HeLa Cancer Cell Growth Inhibition

Objective: Clinical studies show that low intensity (single V/cm), intermediate-frequency (100 kHz-300 kHz) electric fields inhibit the growth of cancer cells, while the mechanism is not yet understood. We examine the hypothesis that electric fields modify the cell membrane potential of dividing cancer cells in a way that correlates with cells growth inhibition. Methods: A Schwan based mathematical model calculates the changes in HeLa cells membrane potential due to single V/cm electric fields and frequencies from 0.1 to 1 MHz. An experimental study examines the effect of these electric fields on the inhibition of HeLa cells growth in an incubator. Results: The theoretical calculation shows that the effects of these electric fields on cell membrane potential decrease with an increase in frequency. The HeLa cells experiments verified the inhibitory effect of these fields on cell growth. The inhibitory effect is decreasing with an increase in frequency, in a way that is similar to the frequency dependent effect of these fields on the cell membrane potential. Conclusions: The superposition of the theoretical results and the experimental results suggest a correlation between the effect of these fields on the cell membrane potential and inhibition of cancer cell growth. It should be emphasized that correlations do not prove causality, however, they suggest an area for future research. Significance: These findings have value for the understanding of the mechanisms of cancer cells growth inhibition with electric fields and suggest an interesting area of research on the interaction between electromagnetic fields and cancer cells.