Molecular Full Adder Based on DNA Strand Displacement

A new method, namely DNA strand displacement (DSD), has recently emerged in the field of biocomputing. DSD uses the toehold domain as a starting point to cover the strand in the migration domain. In this study, a molecular full adder composed of a molecular basic logic gate, including AND and XOR logic gates, is constructed based on strand displacement and Visual DSD. We used Visual DSD to simulate the molecular full adder. The experimental results show that the molecular full adder can be used for addition logic calculation. In addition, it can realize multi-bit addition logic calculation through a multi-stage cascade reaction. The simulation results verified the efficiency and reliability of our multi-bit molecular full adder, and our method can be used to design complex DNA integrated circuits. This research will lay the foundation for the formation of more complex molecular logic circuit structures and functions, as well as provide new coding ideas for various biocomputing related fields.