Proteins and Cytokines of Blood Cells Supernatant and Histological Atypical Cells Markers in the Breast Diseases

The research looks at concentrations of proteins and cytokines in blood cell supernatants as well as expression of histological markers of atypical cells in the breast diseases. Also, it examines the correlation between concentrations of proteins and cytokines and histological markers of atypical cells. It is found that concentration of histidine rich glycoprotein in blood cell supernatants in the invasive carcinoma of no special type is higher compared to benign breast diseases. Furthermore, the research observes the following statistical relationships: negative correlation between concentrations of estrogen receptor-α and expression of integrin β-1; positive correlation between concentrations of histidine rich glycoprotein and expression of type II collagen; positive correlation between concentrations of e-cadherin and expression of e-cadherin; and positive correlation between concentrations of plasminogen activator inhibitor-1 and expression of type II collagen. Cytokines production by blood cells is identified as Index of the influence of polyclonal activators (combination of mitogens). The research shows the difference in the Index of influence on IL-1RA and TNF-α production between benign breast diseases with high-risk and low-risk of malignant transformation, and between benign breast diseases with high-risk of malignant transformation and breast cancer with lymph node metastasis. The Index of the influence of polyclonal activators on IL-1RA production gets higher as disease prognosis worsens. As blood and tumor samples show, patients with benign breast diseases have negative correlation between index of the influence of polyclonal activators on IL-1RA production and expression of type II collagen. Additionally, patients with breast cancer, as well as breast cancer with lymph node metastasis have positive correlation between expression of e-cadherin and spontaneous and polyclonal activator-stimulated IL-1RA production. The results of the research showed that concentrations of histidine rich glycoprotein and IL-1RA in blood cells supernatant and expression of e-cadherin can be used as markers for early diagnosis of benign breast diseases with risk of malignant transformation.