Design and Development of Point of Care Test and Optical Reader for Early Screening of Kidney Related Disorder

Low-cost, paper-based colorimetric assays for early screening of albumin, creatinine and their ratio are developed. The developed methods are non-invasive and require only 10 μl of the urine sample. A reflectance-based optical reader is also developed for the quantification of the albumin and creatinine. The developed method is based on spot-urine testing, which is advantageous when compared to the conventional 24-hr urine collection. The detection range of albumin and creatinine assays is 10-150 mg/dL and 25-400 mg/dL, respectively. The developed assays and optical reader were tested with the chronic kidney diseased patient's samples at KEM Hospital, Mumbai.