Development of a 3D Printed, Self-Insulated, High-Throughput 3D Microelectrode Array (HT-3DMEA)

We have developed a novel two step makerspace-based microfabrication strategy for the realization of high-throughput (HT), self-insulated 3D Microelectrode Arrays (MEAs). Micro-stereolithography (μSLA) based 3D printing technology not only allows for realization of 3D microelectrode geometries but also enables the monolithic integration of all components of the “bio plate” (standard culture wells) to realize the HT, American National Standards Institute (ANSI)/Society for Lab Automation and Screening (SLAS)-compatible geometry in 1 to 768 well configurations. A unique bifacial design having the 3D microelectrodes on the top-face and the traces for electrical connections on the bottom-face enables the realization of self-insulated 3D MEAs after metal ink casting/printing in the 3D printed vertical channels. These HT-3D MEAs are further characterized using optical microscopy, SEM, Impedance Spectroscopy and well-well impedance variations. This approach enables a rapid, accurate, cost-effective, two step scaling up technique to microfabricate HT-3D Microelectrode Arrays in several Multiwell designs compatible with standard, HT assay equipment such as plate readers, robotic handlers and electrophysiological systems. [2020-0138]