Labeled free Malachite Green immunosensor based on chitosan and gold nano particles

An immunosensor for the detection of malachite green in water and fish samples was developed. GNPs with good biocompatibility and good electroconductivity were fabricated onto the surface of a gold electrode (GE). The polyclonal antibody was immobilized on the electrode. Under optimal conditions, the proposed immunosensor exhibited a sensitive response to malachite green in the range of 0.032 ~100 ng/mL, with a detection limit of 10.7 /mL in buffer. Recoveries of malachite green from spiked water and fish samples ranged from 84.2% to 113.8%, indicating that the proposed immunosensor potential can be used for the detection of real samples.